Fig. 5.
Effect of o-vanillin on Gardos channel activity in A23187-treated RBCs from patients with SCD. Oxygenated RBCs were pre-treated with A23187 (10 μM) for 30 min at 37 °C. CLT (5 μM)-sensitive K+ influx in mmol (l cells h)−1 was then measured in the absence or presence of o-vanillin at the concentrations indicated. (a) Gardos channel activity without or with 5 mM o-vanillin in RBCs from HbSS or HbSC individuals, as indicated; (b) concentration dependence of inhibition of Gardos channel activity in RBCs from HbSS individuals. Histograms represent means ± S.E.M., n = 3.