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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Nat Genet. 2014 Apr 20;46(6):618–623. doi: 10.1038/ng.2949

Figure 1. Segmental trisomy orthologous to human chr.21q22 promotes progenitor B cell transformation.

Figure 1

(a) Regions orthologous to human chromosome 21 that are triplicated in Ts1Rhr and Ts65Dn mice or amplified in iAMP21 B-ALL. (b) Progenitor B cells (B220+CD43+) and Hardy subfractions as percentages of bone marrow (BM) cells (n=5–7/group in 2 independent experiments). (c) Subfractions from mixed populations in recipient BM 16 weeks after competitive transplantation (n=5/group). (d) B cell colonies across 6 passages (n=3 biological replicates/genotype representative of 3 independent experiments, mean values shown, *P<0.05, **P<0.01), and bright field microscopy of 3 Ts1Rhr and 3 WT passage 2 cultures (scale bar = 4 mm). (e) Myeloid colonies across 4 passages (n=3 mice per genotype; NS, not significant). (f) Leukemia-free survival of recipient mice after transplantation of Eµ-CRLF2 (C2)/Eµ-JAK2 R683G (J2)/Pax5+/− (P5), with or without Ts1Rhr (Ts1) BM transduced with vector or dominant negative Ikaros (Ik6) (n=8–10 mice/group). (g) Leukemia-free survival of recipient mice after transplantation of BM transduced with BCR-ABL (n=10 mice/group).