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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Nat Genet. 2014 Apr 20;46(6):618–623. doi: 10.1038/ng.2949

Figure 3. Ts1Rhr B cells have reduced H3K27me3 that results in overexpression of bivalently marked genes.

Figure 3

(a) Gene tracks showing occupancy of histone marks at the Plod2 promoter (one of the 50 core Ts1Rhr genes) in reads per million per base pair (rpm/bp). (b) Levels of H3K27me3 in Ts1Rhr and wild-type B cells at regions enriched for H3K27me3 in wild-type cells (***P<1e-16). (c) Histone marks at the promoters of genes that are upregulated or downregulated in Ts1Rhr vs. wild-type cells (**P< 1e-5). (d) Chromatin marks in wild-type B cells present at promoters of all genes (left) or genes that are upregulated in Ts1Rhr B cells (right, ***P<0.0001 compared to all genes by Chi-square with Yates’ correction). (e) Colony counts in the presence of DMSO or GSK-J4 (n=3–6 biological replicates per genotype, *P<0.05 compared to DMSO for same genotype). (f) Colony counts in the presence of GSK-126 or after withdrawal at passage 5 (*P<0.05 compared to GSK-126 for same genotype, #P<0.05 compared to other genotype or no withdrawal). Arrow indicates GSK-126 withdrawal. (g) Western blotting of passage 2 colonies after 14 total days in culture with DMSO, 1 µM GSK-J4, or 1 µM GSK-126. (h) Western blotting of colonies one passage (7 days) after continuation (+) or removal (−) of GSK-126.