Table 2.

Plasmids used in this study

Plasmid	Description	Source/Reference
pUG6	Vector containing the loxP-kanMX-loxP deletion cassette	Güldener et al. (1996)
YEp357R	2μ vector with lacZ reporter gene, URA3	Myers et al. (1986)
YEp363	2μ vector with lacZ reporter gene, LEU2	Myers et al. (1986)
pSEYC102	CEN vector with lacZ reporter gene, URA3	Emr et al. (1986)
GFP-Yap1p	pRS316 (CEN, URA3), Yap1p fused to GFP	Coleman et al. (1999)
pYES2	2μ vector with GAL1 promoter, URA3	Invitrogen
pGAL-GFP-Yap8p	GFP fused to N terminus of YAP8 in pYES2	This work
pEM16	ACR2-lacZ fusion in YEp357R	This work
pEM19	ACR3-lacZ fusion in pSEYC102	This work
pRW11	ACR3-lacZ fusion in YEp363	This work
pRW3	ACR3 in pFL39 (CEN, TRP1)	Wysocki et al. (1997)
pRW33	ACR3 behind its own promoter lacking the TTAATAA sequence (mutACR3) on pRW3	This work
pEM18	mutACR2-lacZ fusion lacking the TTAATAA sequence in YEp357R	This work
pEM20	mutACR3-lacZ fusion lacking the TTAATAA sequence in pSEYC102	This work
GFP-YAP8-C132A	C132A point mutation in pGAL-GFP-Yap8p (pYES2)	This work
GFP-YAP8-C274A	C274A point mutation in pGAL-GFP-Yap8p (pYES2)	This work
GFP-YAP8-C132A C274A	C132A C274A point mutations in pGAL-GFP-Yap8p (pYES2)	This work
pRS cp-GFP HA YAP1	GFP-fused Yap1p (wt) in pRS314 (CEN, TRP1)	Kuge et al. (1997)
pRS cp-GFP HA yap1 cm46A5	GFP-fused Yap1p-TAT (C598T C620A C629T) in pRS314	Kuge et al. (1997)
pRS cp-GFP HA yap1 3Cys	GFP-fused Yap1p-3Cys (C303T C310T C315T) in pRS314	Kuge et al. (2001)
pRS cp-GFP HA yap1 3Cys, C620A	GFP-fused Yap1p-3Cys C620A (C303T C310T C315T C620A) in pRS314	Kuge et al. (2001)
YEp195-GFP-YAP8	GFP-YAP8 controlled by the endogenous YAP8 promoter in YEplac195 (2μ, URA3)	This work
pJAW96	YCF1-lacZ fusion in pSEYC102	Wemmie et al. (1994)
pAW14	GSH1-lacZ fusion in pSEYC102	Wu and Moye-Rowley (1994)
pCM188	CEN vector, URA3, tetO-CYC1 promoter	Gari et al. (1997)
pMiT004	HIS6-FLAG-tagged YAP8 in pCM188	This work