Table 4.
Control of YCF1-lacZ and GSH1-lacZ expression in metalloid-exposed cells
|
YCF1-lacZ/pSEYC102 (U/OD600)a
|
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| Strain | No stress | As(III)b | As(V)b | Sb(III)b | |||
| Wild-type | 5.4 ± 0.8 | 5.6 ± 0.3 | 5.2 ± 0.8 | 5.4 ± 0.2 | |||
| yap1Δ | 5.2 ± 0.1 | 4.1 ± 0.3 | 4.2 ± 0.3 | 3.6 ± 0.7 | |||
| yap8Δ | 6.1 ± 0.4 | 16.7 ± 0.9 | 13.9 ± 0.4 | 5.0 ± 0.6 | |||
| yap1Δ yap8Δ | 4.6 ± 0.5 | 4.8 ± 1.0 | 5.9 ± 0.4 | 3.5 ± 0.1 | |||
| acr3Δ | 5.1 ± 0.6 | 10.7 ± 0.4 | 8.6 ± 0.3 | 6.3 ± 1.1 | |||
| yap8Δ acr3Δ | 5.0 ± 0.15 | 15.9 ± 0.2 | 13.5 ± 0.45 | 5.9 ± 0.2 | |||
| yap1Δ acr3Δ | 4.0 ± 0.5 | 5.6 ± 1.0 | 6.1 ± 0.7 | 4.35 ± 0.4 | |||
|
GSH1-lacZ/pSEYC102 (U/OD600)a
|
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| Strain | No stress | As(III)b | As(V)b | Sb(III)b | |||
| WT | 10.3 ± 0.6 | 15.0 ± 0.9 | 14.5 ± 1.3 | 18.3 ± 1.1 | |||
| yap1Δ | 7.0 ± 1.4 | 8.4 ± 1.5 | 9.4 ± 1.5 | 10.0 ± 1.1 | |||
| yap8Δ | 11.3 ± 0.7 | 32.8 ± 3.1 | 29.1 ± 2.1 | 20.2 ± 0.9 | |||
| yap1Δ yap8Δ | 7.6 ± 1.0 | 15.3 ± 1.7 | 13.5 ± 2.9 | 12.1 ± 1.5 | |||
| acr3Δ | 11.3 ± 1.2 | 32.9 ± 3.1 | 28.9 ± 3.4 | 21.0 ± 1.8 | |||
| yap8Δ acr3Δ | 11.9 ± 0.7 | 34.9 ± 2.9 | 29.5 ± 4.3 | 20.8 ± 1.9 | |||
| yap1Δ acr3Δ | 6.7 ± 0.7 | 13.3 ± 1.4 | 11.7 ± 1.9 | 10.7 ± 0.9 | |||
a
β-Galactosidase activities are the average of three independent experiments, including standard deviation.
b
YCF1-lacZ and GSH1-lacZ expression from the pSEYC102 centromeric plasmid after 20 h of metalloid preincubation (0.1 mM) followed by 2 h of induction with 1 mM respective metalloid.