Table 2.

Plasmids used

Name	Genea	Promoter	Featuresb	Use	Source
pYS-GAL104	HSP104	GAL1	CEN, URA3	Original screen	Sanchez and Lindquist 1990
pLA1	none	GAL1	CEN, HIS3	Vector control for pGALSc104	L. Arwood and S. Lindquist
pGALSc104	HSP104/mutants	GAL1	CEN, HIS3	Directed mutants	See text
pRS316:GAL1-10	none	GAL1	CEN, URA3	Vector control for 104b-U1	J. Taulien and S. Lindquist
I04b-U1	HSP104/mutants	GAL1	CEN, URA3	Directed mutants	See text
pRS315:GAL1-10	none	GAL1	CEN, LEU2	Vector control for HSP104-b/Leu	J. Taulien and S. Lindquist
HSP104-b/Leu	HSP104/mutants	GAL1	CEN, LEU2	Suppressor screen	Ter-Avanesyan et al., 1993
TM104b	HSP104/mutants	MET14	CEN, TRP1	Alternative induction system	E. Schirmer and S. Lindquist
GAL4.ER.VP16	GAL4/ER/VP16	ADH	CEN, HIS3	Estradiol induction of GAL1 plasmids	Louvion et al., 1993
pEMBL-Δ3AUG	SUP35 C-terminus	SUP35	2μm, URA3	Test suppression	Ter-Avanesyan et al., 1993
pRS303-LacZ	LacZ	GAL1	none, HIS3	Integration into suppressor strain	See text
CDC12-WT	CDC12/mutants	CDC12	CEN, URA3	Suppressor screen	See text
GAL-CDC12	CDC12/mutants	GAL1	CEN, URA3	Suppressor screen	See text
GFP-CDC3	GFP-CDC3	CDC3	CEN, URA3	Septin visualization	M. Longtine
GFP-CDC12	GFP-CDC12	CDC12	CEN, URA3	Septin visualization	See text

^aPlasmids containing directed mutants are indicated in the text by the plasmid name with the mutation in parentheses, e.g., pGALSc104 carrying a mutant G217S/T499I allele is designated pGALSc104(G217S/T449I).

^bFeatures listed are yeast replication (CEN is low copy number and 2μm is high copy number) and auxotrophic selection.