Figure 8.

Inhibition of the removal of the last Glc extends the association of H2a precursor to CNX and of cleaved sH2a to ERp57. (A) Cells expressing H2a were metabolically labeled for 40 min and chased for the indicated times in the absence or in the presence of 150 μg/ml CST. In this experiment, CST was present only during the chase period. Cell lysates were immunoprecipitated as in Figure 2. The fluorographies in the left and middle panels were exposed 4 times longer than that in the right panel. The experiment is representative of three repeat experiments. (B) Values from PhosphorImager quantitation of the experiment in A are plotted for H2a bound to ERp57 or CNX relative to the pulse (= 100).