Table 1.
Nucleotide sequence of the primers used in this study
| Gene | Sequence (5′-3′) | |
|---|---|---|
| 1 |
Pdx1 F |
GGAGCCGGAGGAGAACAAG |
| Pdx1 R |
CTCGGTCAAGTTCAACATGACAG |
|
| 2 |
Pax4 F |
CAGCGCTGCTGGACTT |
| Pax4 R |
CAGCGCTGCTGGACTT |
|
| 3 |
Insulin F |
ACCAGCATCTGCTCCCTCTA |
| Insulin R |
GGTTCAAGGGCTTTATTCCA |
|
| 4 |
Ngn3 F |
CTATTCTTTTGCGCCGGTAGA |
| Ngn3 R |
CTCACGGGTCACTTGGACAGT |
|
| 5 |
Glucagon F |
CCCAAGATTTTGTGCAGTGGTT |
| Glucagon R |
CAGCATGTCTCTCAAATTCATCGT |
|
| 6 |
Foxa2 F |
CTGAGCGAGATCTACCAGTGGA |
| Foxa2 R |
CAGTCGTTGAAGGAGAGCGAGT |
|
| 7 |
Sox17 F |
GCATGACTCCGGTGTGAATCT |
| Sox17 R |
TCACACGTCAGGATAGTTGCAGT |
|
| 8 | GAPDH F |
GCACCGTCAAGGCTGAGAAC |
| GAPDH R | TGGTGAAGACGCCAGTGGA |
F: forward primer; R: reverse primer.