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. 2014 May 11;11:22. doi: 10.1186/1743-8977-11-22

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Copyright © 2014 Luanpitpong et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Isolation and characterization of CSC-like cells from SWCNT-exposed lung epithelial cells. (A) SP and non-SP (NSP) FACS sorting profiles of malignant transformed BSW and lung cancer H460 cells. Due to their highly dynamic nature of CSC, cells were freshly isolated prior to each experiment. (B) Light scattering properties of SP (red) and NSP (blue) cells derived from BSW and H460 cells as evaluated by flow cytometry. (C, D) Analysis of colony formation (C) and tumor spheres (D) in SP and NSP cells derived from BSW and H460 cells after two weeks of culture.