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. 2014 May 11;11:22. doi: 10.1186/1743-8977-11-22

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Copyright © 2014 Luanpitpong et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Aggressive tumor phenotypes of CSC-like cells derived from SWCNT-exposed lung epithelial cells. (A) Acquired apoptosis resistance of SP cells derived from malignant transformed BSW and lung cancer H460 cells to chemotherapy cisplatin (100 μM), doxorubicin (1 μM), antimycin A (30 μM), and etoposide (500 nM), compared to their respective non-SP (NSP) cells, as analyzed by Hoechst 33342 assay at 24 hours post-treatment. (B) Cell invasion (top) and migration (bottom) assays of SP and NSP cells derived from BSW and H460 cells, and the passage control BC cells, at 48 hours after incubation. Plots are relative invasion and migration level normalized to passage control BC cells. Representative fluorescence micrographs of invading and migrating cells are shown (right). Data are means ± SD (n = 4). *p < 0.05 vs. SP cells.