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. 2014 May 11;11:22. doi: 10.1186/1743-8977-11-22

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Copyright © 2014 Luanpitpong et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Expression of stem cell markers in CSC-like cells derived from SWCNT-exposed lung epithelial cells. (A) Analysis of pluripotent stem cell markers in SP and non-SP (NSP) derived from malignant transformed BSW cells using the Proteome Profiler™ Array (R&D Systems). Pixel densities of remarkable stem cell markers (1) Nanog, (2) SOX-2, (3) E-cadherin (E-Cad), and (4) SOX-17 from the arrays are shown. (B) Western blot analysis of Nanog, SOX-2, E-cadherin, and SOX-17 comparing SP and NSP cells derived from BSW cells. (C, D) Analysis of stem cell surface markers CD24, CD44, and CD133 in SP and NSP cells derived from BSW cells using flow cytometry, comparing with the passage control BC cells. Plots are relative fluorescence level normalized to control BC cells. Data are means ± SD (n = 3). *p < 0.05 vs. SP cells, ^#p < 0.05 vs. BC cells.