Figure 3.

On-going palmitoylation of FIMs regulates filopodia formation in COS-7 cells. (A) COS-7 cells transfected with the GAP-43 palmitoylation motif and treated with 50 μM 2-bromopalmitate (24 h posttransfection), which blocks protein palmitoylation, show a significant decrease in filopodia outgrowth. (B) Graph shows the percentage of cells expressing filopodia for the different treatments; untreated and palmitate-treated cells are statistically different from 2-bromopalmitate-treated cells (**p < 0.01; ***p < 0.001). Scale bar, 10 μm.