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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: J Struct Biol. 2014 Mar 12;186(3):412–419. doi: 10.1016/j.jsb.2014.03.004

Figure 1.

Figure 1

Schematic representation of the recombinant DNA strategy used to make spider silk block copolymers for the study. NcoI, NheI, SpeI, XhoI are restriction enzymes (RE). A box above/below a specific RE indicates an oligonucleotide sequence recognized by this RE. pet30a(+) plasmid is in blue, cloning linker is in yellow, silk hydrophobic block A is in magenta, and silk hydrophilic block B is in green. Steps are the following: (1) digestion, (2) ligation of a linker, (3) ligation of A block, (4) ligation of B block.