Fig. 7.
Effects of cholesterol depletion and loading on the partition of TrkB receptors to lipid rafts and on adenosine A2A receptor binding properties. Cultured cortical neurons were treated with/without 20 nM CGS 21680 for 30 min after incubation with 3 mM MβCD or MβCD–cholesterol complexes (water-soluble cholesterol, wsCLT, 50 μg/ml cholesterol). a, b Lipid raft (fraction #2) analysis of the effect of MβCD on TrkB sublocalization after CGS 21680 treatment. MβCD–cholesterol complexes were used to load cholesterol to membranes. c Cholesterol content in lipid raft (#2) and non-raft fractions (#8) [56] in control conditions and after 20 nM CGS 21680 incubation for 30 min. d Saturation curves for the specific binding of the A2A receptor antagonist [3H] ZM 241385 in cortical membranes from cells in control conditions (open circles), after incubation with MβCD (closed squares) or with wsCLT (closed triangles) treatment. Data are expressed as mean ± SEM of three to five independent experiments. *p < 0.05, compared to 100 %