Figure 3.

Subcellular localization of endogenous Rab14 protein. NRK cells were fixed with 3% PFA, permeabilized with saponin, and costained using rabbit anti-Rab14 antibody (B and E) and mouse monoclonal antibodies against TfR (A), GM130 (D), followed by incubation with Alexa-488–labeled anti-rabbit and Alexa-594–labeled anti-mouse IgG antibodies. (C and F) Merged images of A and B and D and E, respectively. Bar, 10 μm. (G) Ultrathin cryosection of an NRK cell, immunogold-labeled for Rab14 (10-nm gold). Rab14 is present on the Golgi stack (G) and associated vesicles, in vesicles (arrows) nearby early EEs and in more dispersed tubulo-vesicular structures (arrowheads). Bar, 200 nm.