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. 2004 May;15(5):2218–2229. doi: 10.1091/mbc.E03-10-0777

Figure 4.

Figure 4.

Detection of overexpressed GFP-Rab14 fusion proteins in live NRK cells by fluorescence microscopy. Live NRK cells were imaged using GFP fluorescence after 24 h of transfection with various GFP-Rab14 constructs. (A) GFP-Rab14wt. (B and C) GFP-Rab14Q70L. (D) GFP-Rab14S25N. (E) GFP-Rab14N124I. Large vesicles were formed in GFP-Rab14 wt and Q70L cells. The GFP signal was more diffuse and localized to perinuclear Golgi-like structures in GFP-Rab14S25N and N124I cells. Note that the cell shown in B expresses a moderate-to-high level of GFP, whereas that in C expresses low-to-moderate level. Bar, 10 μm.