Cell rupture-related ATP release. a Scratching the cells with a needle resulted in rupture of both cell bodies and dendrites. Upper panel, before scratch; lower panel, after scratch. b Cells were scratched with a needle zero, one, three, five and seven times, which led to release of ATP depending on the number of scratches (n = 7). Arrow indicates ruptured dendrites or cell bodies, triangle represents ATP release in each experiment, broken line represents mean ATP release and three asterisks represent p ≤ 0.001