Figure 1.

Cdc6 is required for premeiotic DNA replication. MATa/MATα cdc6-1/cdc6-1 (strain Y208, top two panels) and its isogenic cdc6–1/CDC6 (strain Y1466, bottom panel) cells were grown at 25°C in PSP2 to a titer of 1 × 10⁷ cells/ml. Cells were washed in water, resuspended in warm SPM, and incubated at either 25 or 35°C (time 0) (top). Nocodazole was added to the PSP2 culture to a final concentration of 10 μg/ml. After 2-h incubation, cells were washed and resuspended in SPM as described above (time 0). Samples were taken at 2 h (dashed gray line) and 48 h (solid black line) to process for FACS analysis.