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. 2004 May;15(5):2264–2275. doi: 10.1091/mbc.E03-10-0772

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Figure 5. — Immunofluorescence localization of melanosomes, Rab27a, and cytoskeletal proteins. Wild-type (A, B, and D–F) and ashen (C) RPE and choroid were labeled with fluorescent phalloidin (red in A–C, E, and F), antitubulin (green in A–C, red in D), anti-Rab27a (green in D), antimyosin VIIa (green in E), and antimyosin Va (green in F). Melanosomes identified by transmitted light have been artificially colored blue in the merged images. Arrows indicate examples of coincidence between melanosomes and rab27a (D) and melanosomes and myosin VIIa (E). AP, apical processes; CB, cell body; BI, basal infoldings. Bar, 5 μm.