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. Author manuscript; available in PMC: 2015 Mar 21.
Published in final edited form as: Chem Commun (Camb). 2014 Mar 21;50(23):3027–3029. doi: 10.1039/c3cc00173c

Fig. 3.

Fig. 3

G Neo B inhibits miR-10b biogenesis and boosts production of a downstream target. A, miR-10b is processed to produce the mature miRNA in two steps, first by Drosha in the nucleus and then by Dicer in the cytoplasm. The mature miRNA modulates protein expression. The target site for G Neo B is highlighted with a purple circle. B, G Neo B inhibits production of mature miR-10b as determined by qRT-PCR. C, G Neo B increases the amount of pri-miR-10b and diminishes pre- and mature miR-10b levels, as expected if G Neo B binds to the Drosha site and inhibits processing. D, G Neo B affects production of luciferase when a luciferase mRNA is under control of miR-10b, validating that G Neo B affect miRNA biogenesis and downstream protein targets (*, p < 0.05; **, p < 0.01 as determined by a two-tailed student t-test; n≥3).