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Absorption data for O2 and H2O2 binding in pMMO. (Left) H2O2 reacted with solubilized pMMO from M. capsulatus (Bath) (Heme contaminant absorbs at 410 nm). Difference absorption spectra for both H2O2 and O2 reacted with (Middle) spmoB and (Right) O2 with spmoB and the spmoB_H48N, spmoB_H137,139A, and spmoB_H148N_137,139A variants. All samples were anaerobically reduced before addition of oxidant. (Reprinted with permission from Ref. 717. Copyright 2012 American Chemical Society.)
