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Resonance Raman spectra and difference spectra of the primary oxygen intermediate from the reaction of fully reduced CcO with dioxygen. Reacion of fully reduced with A) 16O2, B) 18O2, C) difference spectra showing the isotopic shift and D) difference spectra showing isotopic shift of prmary intermediate in the reaction of oxygen with the mixed-valence (Mixed Val) enzyme. Adapted from Reference1035 Proc Natl Acad Science USA
