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. Author manuscript; available in PMC: 2015 Apr 9.
Published in final edited form as: Chem Rev. 2014 Mar 3;114(7):3659–3853. doi: 10.1021/cr400327t

Table 29.

Steady-State Kinetic properties for multicopper oxidases

Enzyme Km
(mM)		 kcat
(s−1)		 Substrate pH Referenceb
Organic Oxidases
Laccase (Tree) R. vernicifera 200 560 Hydroquinone 7.5 852
Laccase (SLAC) S. coelicolor 3.6 5.8 2,6-DMPa 7.0 858
Laccase (Fungal) T. villosa 0.13 25.2 Hydroquinone 5.0 859
M. thermophila 0.73 15.5 Hydroquinone 5.0 859
CotA B. licheniformis 0.0567 28 2,6-DMPa 7.0 854
B subtilis 0.216 29 2,6-DMPa 7.0 855
Ascorbate Oxidase C. Pepo medullosa 0.200 7.50 × 103 Ascorbate 6.0 & 7.5 856
Metalloxidases
Fet3p S. cerevisiae 0.0054 1.01 Fe(II) 5.0 843
Ceruloplasmin H. sapien 0.0083 0.51 Fe(II) 5.0 843
CueO E. coli 0.165 15.2 Cu(I) 5.0 853
a

2,6-dimethoxy-phenol;