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Various 2,4-QD substrates and steady-state kinetics parameters for 2,4-dioxygenation by P. olsonii 2,4-QD (obtained with 20% DMSO assay content).1112
| Substrate structure | kcat (s−1) | KM (µM) | kcat/KM (M−1 s−1) | |
|---|---|---|---|---|
| quercetin |  |
167±3 | 19±1 | 8.8×106 |
| kaempferol |  |
467±17 | 13±2 | 3.6×107 |
| galangin |  |
33±5 | 20±4 | 1.7×106 |
| fisetin |  |
57±7 | 85±16 | 6.7×105 |
