Table 41.
Selected parameters for crystallographically characterized nitrite-bound species in the Cu dependent nitrite reducases.
| Source Organism |
Mutations | Resolution (Å) |
pH | Cu-N Distance (Å) |
Cu-O Bond Lengths (Å) |
PDB ID |
|---|---|---|---|---|---|---|
| Oxidized CuNiR + Nitrite | ||||||
| A. xylosoxidans | WT | 2.80 | 6.0 | 2.484 | 1.732, 2.376 | 1nds1258 |
| A. xylosoxidans | N90S | 3.00 | 7.4 | 2.488 | 2.000, >2.367 | 2xx11255 |
| A. xylosoxidans | H313Q | 1.72 | 7.1 | 2.406 | 1.861, >2.419 | 1wa21259 |
| A. faecalis | WT | 1.40 | 7.0 | 2.360 | 2.039, >2.290 | 1sjm1260 |
| A. faecalis | WT | 1.80 | 7.0 | 2.351 | 2.165, >2.404 | 1as61250 |
| R. sphaeroides | WT | 1.85 | 8.4 | 2dws1256 | ||
| R. sphaeroides | WT | 1.90 | 6.0 | 2dwt1256 | ||
| N. gonorrhoeae | WT | 1.95 | 7.0 | 2.313 | 2.022, >2.678 | 1kbv1261 |
| A. cycloclastes | WT | 2.20 | 5.4 | 2.697 | 2.157, >2.530 | 1nid1262 |
| A. faecalis | D98N | 1.65 | 7.0 | 2.498 | 2.289, >2.475 | 1j9q1263 |
| A. faecalis | H255N | 1.90 | 7.0 | 3.2 | 2.111 | 1j9s1263 |
| A. faecalis | I257A | 1.70 | 7.0 | 3.3 | 1.935 | 1l9o1264 |
| A. faecalis | I257G | 1.75 | 7.0 | 2.285 3.3 |
2.036, >2.114 2.096 |
1l9p1264 |
| Reduced CuNiR + Nitrite | ||||||
| A. faecalis | WT | 1.85 | 7.0 | 2.458 | 2.229, >2.674 | 1as81250 |
| Reduced CuNiR + NO | ||||||
| A. faecalis | WT | 1.58 | 7.0 | 2.4 | 1.982, >2.4 | 2ppc1265 |
| Endogenous Nitrite | ||||||
| A. cycloclastes | WT | 1.15 | 6.5 | 2.154 | 1.978, >2.190 | 2bwi1246 |