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. 2009 Nov 6;101(3):707–712. doi: 10.1111/j.1349-7006.2009.01425.x

Figure 3.

Figure 3

 Inhibitor of DNA binding 2 (ID2) overexpression rescues Du145 cells from apoptosis. (A) Semiquantitation of ID2 expression by RT‐PCR on RNA from Du145 cells transfected with pCMVscript (lanes 1 and 2) or pCMV‐ID2 (lanes 3 and 4). cDNA templates (200 ng) from Du145 transiently transfected with pCMV script or pCMV‐ID2 were titrated to the indicated dilutions. PCR were carried out using primers specific for ID2 or β‐actin. The PCR products were then electrophoresed and visualized in 2% agarose gel. (B) Representative samples of FACS analysis of Du145 transfected with pCMV‐ID2 or pCMVscript, and treated with or without (−)‐epigallocatechin‐3‐gallate (EGCG). (C) Means of DU145 cell deaths by FACS of (B). Each condition was triplicated in the analysis. Standard deviations are indicated.