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. 2009 Nov 6;101(3):707–712. doi: 10.1111/j.1349-7006.2009.01425.x

Figure 4.

Figure 4

 Suppression of inhibitor of DNA binding 2 (ID2) in Du145 cells induces cell death. (A) Immunoblotting analysis of ID2 expression in Du145 cells. Protein extracts from Du145 cells transfected with the indicated morpholino oligonucleotides were electrophoresed in 7% SDS‐PAGE gel and immunobloted with antibodies specific for ID2 and β‐actin. (B) Representative samples of FACS analysis of Du145 cells transfected with morpholino oligonucleotides specific for ID2 (mID2) and scramble control (Scr). (C) Means of DU145 cell deaths by FACS quantification of Du145 cells transfected with Scr control or mID2. Each condition was carried out in triplicate. Standard deviations are indicated.