Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Reconstitution of GR nuclear import in vitro. Bar, 10 μM. (A) GFP-GST-407-525 (0.5 μM) was incubated with permeabilized cells, 2 mg/ml HeLa extract, ATP mix, 6 μM RanQ69L, or apyrase for 30 min at 30°C or 4°C, as indicated. (B) Purified GR (30 nM) was incubated with digitonin-permeabilized cells, 2 mg/ml HeLa extract, ATP mix, 6 μM RanQ69L, or apyrase for 30 min at 30°Cor4°C, as indicated.
