Figure 6.

Importin 7 and the importin α-importin β heterodimer potentiate the import of full-length rat GR. Bar, 10 μM. (A) GR (30 nM), ATP mix, 3 μM RanGDP, and 2 μM importin 7, 2 μM importin 8, 4 μM importin α,or1 μM importin β, as indicated, were incubated with digitonin permeabilized cells for 30 min at 30°C. (B) GR (30 nM) was incubated with permeabilized cells, ATP mix, and the indicated concentration of HeLa cell extract. (C) GR (30 nM), ATP mix, 0.3 mg/ml HeLa extract and 2 μM importin 7 or 4 μM importin α were incubated with digitonin permeablized cells as above.