Figure 8.

Models for Gfh1p and Mbo1p functions. (A) Gfh1p might play a specialized role as part of a γ-TuC cap structure at the cytoplasmic face of the SPB. This aids proper anchoring of astral microtubules to the SPB (top cell). In the absence of Gfh1p function (bottom cell), the cap would be altered resulting in the detachment of astral microtubules from the SPB. (B) During mitosis, Mbo1p localizes to a discontinuous ring at the medial region (top cell) and recruits other members of the γ-TuC (middle cell). The assembled complex might help facilitate the coalescence of the ring to an EMTOC and nucleate the PAA (bottom cell).