Table 1.
Cl– transport rates of WT and mutant CLC-ec1.
| Protein | γ (ion s–1) | γHg (ion s–1) | γHg/γ | n | nHg | Crosslinked Helices |
|---|---|---|---|---|---|---|
| WT | 2337±378 | 1341±224 | 0.57±0.19 | 6 | 8 | -- |
| L252C P424C | 694±77 | 528±48 | 0.76±0.15 | 7 | 7 | J-Q |
| A392C M425C | 1136±66 | 753±97 | 0.66±0.12 | 7 | 7 | O-Q |
| A392C T428C | 1734±229 | 1177±235 | 0.68±0.23 | 7 | 7 | O-Q |
| A396C T428C | 811±96 | 394±59 | 0.49±0.13 | 7 | 7 | O-Q |
| A396C G429C | 714±90 | 500±77 | 0.70±0.20 | 6 | 4 | O-Q |
| A399C A432C | 1357±87 | 16±36 | 0.01±0.03 | 10 | 10 | O-Q |
| G259C A399C | 552±83 | 32±19 | 0.06±0.04 | 6 | 6 | J-O |
| A399C A396C | 680±26 | 227±46 | 0.33±0.08 | 6 | 6 | O-O |
| G259C A432C | 517±78 | 320±70 | 0.62±0.23 | 15 | 15 | J-Q |
| A399C A432C E148A Y445A | 23260±1480 | 22255±2191 | 0.96±0.16 | 4 | 3 | O-Q |
| A399C A432C Y445A | 218±37 | 72±21 | 0.33±0.15 | 12 | 11 | O-Q |
| A399C A432C E148A | 564±26 | 244±21 | 0.43±0.06 | 9 | 9 | O-Q |
| A399C A432C I402G | 909±215 | 572±65 | 0.63±0.22 | 6 | 6 | O-Q |
| A399C A432C I402A | 668±57 | 248±92 | 0.37±0.17 | 6 | 6 | O-Q |
| A399C A432C I402S | 967±137 | 629±54 | 0.65±0.15 | 8 | 8 | O-Q |
| G259C | 257±57 | -- | -- | 4 | -- | -- |
| A399C | 1652±160 | -- | -- | 3 | -- | -- |
| A432C | 1268±77 | -- | -- | 4 | -- | -- |
Cl– transport rates measured using the efflux assay before (γ) and after Hg2+ treatment (γHg). Reported values are the average ± s.e.m.; n and nHg are the number of repeats for the untreated and treated mutants respectively, each from at least 2 independent preparations.