Figure 5.

CAP1 is important for cell migration. (A and Supplementary Videos 1 and 2) Representative examples of motilities of wild-type (top) and CAP1 knockdown (bottom) B16F1 cells expressing GFP-actin. White arrows indicate the locations of the nuclei in the first frame. Wild-type cells typically displayed directional migration. CAP1 knockdown cells were capable to extend and retract their lamellipodia, but they were generally unable to migrate to certain direction. Note that also the reorganization of actin filament structures is much slower in CAP1 knockdown cells than in wild-type cells. Bar, 10 μm. (B) Migration of 44 wild-type and 28 CAP1 knockdown B16F1 cells were monitored for 100 min. The average motility distances of wild-type cells and CAP1 knockdown cells were 53.4 and 27.6 μm, respectively. SEM and statistical significance of the data are indicated in the graph.