Figure 6.

Model for ubiquitination and intracellular transport of Pma1-7. Pma1-7 acquires ubiquitination (in grey) after ER export and before entry into the endosomal pathway. In mutants blocked in various steps of the endosomal pathway (vps8, vps45, pep12) before deubiquitination at the vacuole, Pma1-7 ubiquitination is increased. In the absence of ubiquitination in rsp5-1 and bul1 bul2, Pma1-7 (in black) is delivered to the plasma membrane. In vps1 and gga1 gga2 cells, ubiquitinated Pma1-7 is diverted to the plasma membrane where it undergoes deubiquitination (in black).