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. Author manuscript; available in PMC: 2014 Dec 19.
Published in final edited form as: Oncogene. 2013 Jul 22;33(25):3316–3324. doi: 10.1038/onc.2013.290

Figure 2.

Figure 2

The PHOX2B-HPCAL1 interaction results in nuclear translocation of HPCAL1. (a) GFP-tagged WT PHOX2B and mCherry-tagged HPCAL1 were transfected into HeLa cells. 48 hours later, the cells were fixed in 4% formaldehyde in PBS and mounted on ProLong Gold antifade reagent with DAPI (Invitrogen). Images were taken on a Nikon Eclipse Ti confocal microscope (Melville, NY) and processed with ImageJ software (40X magnification). (b) GFP-tagged WT and the indicated mutant GFP-tagged PHOX2B genes were cotransfected with HPCAL1-mCherry and imaged as in panel (a).