Figure 9.

Controls for a commercially-available cytotoxicity assay that monitors cell wall lysis with a luminescent readout (a). Bacteria treated with two antibiotics, only one utilizing a mechanism of action that interferes with peptidoglycan synthesis, shows distinct luminescent dose responses. Cell lysis data for S aureus and B. subtilis cultures treated for 24 hours with compound 4 (b). Luminescence is measured in arbitrary units, corrected for the cell density of the sample (variable depending on growth inhibition), and presented as a percentage of untreated cell luminescence.