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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Apr 2;1839(6):493–505. doi: 10.1016/j.bbagrm.2014.03.014

Figure 7. A TRE binding site influences ARE-driven enhancer activity.

Figure 7

Figure 7

A.) TPA treatments down-regulated the enhancer activity in A549 cells. Cells were transfected with the E1b −300 or E1b DNase I HS-2/E1b-300 plasmids for 18hr, treated with TPA for 6hr and harvested for luciferase assay. *p<0.05 compared to DMSO controls. B.) Western blot analysis of mEH expression in A549 cells after treated with TPA for 24 and 48 h. GAPDH was used as a loading control. C.) Regulation of the E1b DNase HS-2 enhancer activity by JUN overexpression in A549 cells. Cells were co-transfected with the JUN expressing plasmid and the reporter vectors containing E1b-300 promoter or E1b DNase I HS-2/E1b-300 promoter. After 24 h, luciferase activity was measured. *p<0.05 compared to empty vector controls. D.) JUN blocks tBHQ induced activation of E1b DNase I HS-2 enhancer activity in BEAS-2B cells. Cells were co-transfected with the JUN expressing plasmid and the reporter vectors containing E1b-300 promoter or E1b DNase I HS-2/E1b-300 promoter for 18 h and treated with DMSO or tBHQ for 6 h before harvested for luciferase assay. *p<0.05 compared to DMSO controls. All luciferase values represent mean ± SD.