Figure 3.

Immunofluorescence localization of GOS-28 and GM130 in CHO, ldlB, and ldlC cells treated without and with lactacystin. (A) Cells were incubated for 8 h without (none) or with (+Lact) 25 μM lactacystin, fixed, and stained with antibodies to GOS-28 (red) and GM130 (green). To visualize more clearly the distribution of GOS-28 by using confocal microscopy, the images from ldlB and ldlC cells (weaker signals) were collected with higher signal gains than those from CHO cells. However, a fixed signal gain was used to collect images from any given cell type when comparing the effects of incubation without or with lactacystin. (B) The ldlB cells were preincubated with cycloheximide (+CHX, 140 μg/ml) for 1 h and then further incubated for 8 h without or with (+Lact) 25 μM lactacystin in the presence of the cycloheximide. Bar, 10 μm.