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. 2004 May;15(5):2423–2435. doi: 10.1091/mbc.E03-09-0699

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Copyright © 2004, The American Society for Cell Biology

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Figure 4. — Steady-state levels of GOS-28, GS15, the subunits of COG and GM130 in untransfected and COG subunit-overexpressing CHO and ldlB cells. Total cell lysates from the CHO, ldlB, and ldlB[COG1] cells, as well as transfected CHO cells that overexpress exogenous Cog1, Cog2, or HA-tagged Cog7 (CHO[COG1], CHO[COG2] and CHO[HA-COG7]) were subjected to SDS-PAGE and immunoblotting. Open arrowhead indicates the HA-tagged Cog7 protein whose electrophoretic mobility is slightly less than that of the endogenous Cog7.