Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Analysis of cell cycle by flow cytometry. Cells overexpressing ghrelin or pcDNA3.1 were harvested after culturing for 48 h, stained with propidium iodide in hypotonic lysis buffer, and analyzed by flow cytometry. DNA histogram was deconvoluted, and percentage of cells in each phase of cell cycle was calculated and expressed as mean ± SEM. Results are representative of four separate experiments. *p < 0.05.
