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No expression of known ghrelin receptor mRNA in 3T3-L1 cells. RT-PCR was performed on total RNA extracted from undifferentiated and differentiated 3T3-L1 cells with primers specific for the known ghrelin receptor (top) or β-actin (bottom). Lane 1, marker; lane 2, undifferentiated 3T3-L1 cells; lane 3, 3T3-L1 cells differentiated for 10 d; lane 4, positive control with RNA from hypothalamus; and lane 5, negative control.
