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. Author manuscript; available in PMC: 2014 Jun 2.
Published in final edited form as: Angew Chem Int Ed Engl. 2009;48(48):9097–9101. doi: 10.1002/anie.200904504

Figure 2.

Figure 2

AAQ is an open-channel blocker of the Sh-IR internal TEA-binding site. a & b) Whole-cell current responses to 200 ms depolarizing voltage steps from −70 to +40 mV in the presence of 380 nm (gray) or 500 nm (black) light. a) Open-channel block is apparent in AAQ-treated Sh-IR channels (Scale bar: 6 nA, 25 ms.) b) Open-channel block is absent in MAQ-treated SPARK channels (Scale bar: 0.5 nA, 25 ms.) c) Steady-state I/V curves under 380 nm (gray), 420 nm (black dashes) and 500 nm (black) illumination. I/V curves from each of 4 cells were normalized to the current measured at 380 nm and +60 mV. d) Dependence of AAQ block on [K+]o. Whole-cell current responses of a cell to depolarizing voltage steps from –70 to +40 mV in the presence of 380 nm (gray) or 500 nm (black) light at the indicated [K+]o. Similar results were obtained in 2 other cells (Scale bar: 5 nA, 50 ms.)