Skip to main content
. Author manuscript; available in PMC: 2015 Mar 31.
Published in final edited form as: Dev Cell. 2014 Mar 6;28(6):697–710. doi: 10.1016/j.devcel.2014.01.028

Figure 2. blmp-1 Impedes Dorsal Migration of the Gonad.

Figure 2

(A) Schematic representation of gonadal migration programs of WT worms and various mutants as inferred from inspection. Arrowheads indicate positions of the dtcs. (B) Representative DIC images of WT and dre-1;daf-12 young adult worms grown on L4440 empty vector control or blmp-1 RNAi, respectively, showing one gonadal arm. Arrowheads indicate the position of the dtc. v = vulva. (C) blmp-1 depletion specifically suppresses gonadal migration defects of dre-1;daf-12 and dre-1;lin-29(n546) mutants. ***p<0.001 (ANOVA). Mean+SEM (n=3, 40 gonadal arms each). (D) Representative DIC images of 27 h post-hatching WT and blmp-1 mutant larvae staged by the vulval precursor cells (arrowheads, upper panel). Dtcs migrate precociously in the blmp-1 mutant (arrowheads, lower panel). (E) In blmp-1 mutants, as well as WT and dre-1;daf-12 mutants grown on blmp-1 RNAi dtcs turn dorsally ahead of time. ***<0.001 (ANOVA). Mean±SD.

ns = not significant. nd = not determined. Scale bars = 10 μm.