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. Author manuscript; available in PMC: 2015 Mar 31.
Published in final edited form as: Dev Cell. 2014 Mar 6;28(6):697–710. doi: 10.1016/j.devcel.2014.01.028

Figure 3. blmp-1 Interacts with Precocious and Retarded Heterochronic Loci.

Figure 3

(A) Epistasis and synergy experiments of blmp-1(tm548)/blmp-1i with precocious (hbl-1(ve18), lin-41i, lin-42(n1089), sop-2(bx91)) and retarded (let-7(n2853ts), lin-29(n546), mab-10(e1248)) heterochronic loci (n>20). L4440 empty vector functions as control. Partial seam fusion refers to an incomplete seam syncytium with remaining unfused seam cells. Partial alae formation refers to poorly formed or incomplete adult alae. Temperature-sensitive alleles of sop-2 and let-7 were observed after shifting L1 larvae from 15°C to 23°C or 20°C, respectively. (B) Representative pictures of seam cell adherence junctions as visualized by ajm-1::gfp in hbl-1 and lin-42 L3 larvae grown on L4440 empty vector control or blmp-1 RNAi, respectively. Arrowheads indicate seam-seam boundaries. (C) Representative DIC images of hbl-1 and let-7 mutants showing adult alae formation under control (L4440) and blmp-1 depleted conditions at the early L4 or young adult (yA) stage. Arrowheads indicate positions of the seams.

Scale bars = 10 μm. See also Figure S1.