Table 1.

Primers used for RT-PCR analysis of gene expression, and PCR experimental condition.

Gene	Direction	Primer sequence : (5′-3′)	Product length (bpa)	Tba(°C)	Cycles
Inhibin α	Forward	TGTCGTCAGGGCAAGAGAACTATG	401	58	31
	Reverse	ACCTGGTGGCTGCGTATGTGT
Inhibin βA	Forward	GGGTAAAGTGGGGGAAAACGGGTATG	412	65	31
	Reverse	GCGCTGGATGCTGCTAGACACTGG
Inhibin βB	Forward	GGCCGGCCCAACATCACG	393	63	31
	Reverse	GTCCACCTTCTTCTCCACCACATTCC
Inhibin βC	Forward	CTCAGCCAGCGCCCCATACTCA	221	63	31
	Reverse	TGCAGGACCTCCACACCACCAGTAG
Inhibin βE	Forward	CTGACACCCCAAGGAGAACG	431	65	31
	Reverse	CCGCTAGAGGGCAGAGTCAG
GAPDH	Forward	TATGACTCTACCCACGGCAAGTTCAA	830	60	21
	Reverse	ACCACCCTGTTGCTGTAGCCATATTCAT

^abp, base pair(s);

^bT_a, annealing temperature.

Nucleotide sequences referred were as follows; BC08564 and M36453 for inhibin α subunit, M37482 for inhibin β_A subunit, M32756-M32758 for inhibin β_B subunit, AF140031 for inhibin β_C subunit, AF089825 and AF140032 for inhibin β_E subunit. Each pair of PCR primers was designed on different exons of the target gene to avoid amplification of genomic DNA.