Table 2.
In vitro effects of pH, lactic and acetic acids on Pseudomonas aeruginosa PAO1 growth
| Acid concentrations (mM) | OD 595nm of P. aeruginosa at different pH and acid concentrations* | |||
|---|---|---|---|---|
| Lactic acid |
pH 7 |
pH 6 |
pH 5 |
pH 4 |
| 0 |
0.60 ± 0.03 |
0.62 ± 0.01 |
0.43 ± 0.02 |
0.01 ± 0.00 |
| 50 |
0.74 ± 0.06 |
0.81 ± 0.11 |
0.47 ± 0.19 |
0.12 ± 0.01 |
| 100 |
0.65 ± 0.02 |
0.65 ± 0.02 |
0.23 ± 0.07 |
0.12 ± 0.01 |
| Acetic acid |
pH 7 |
pH 6 |
pH 5 |
pH 4 |
| 0 |
0.60 ± 0.03 |
0.62 ± 0.01 |
0.43 ± 0.02 |
0.01 ± 0.00 |
| 12.5 |
0.70 ± 0.06 |
0.58 ± 0.02 |
0.38 ± 0.27 |
0.12 ± 0.01 |
| 25 |
0.63 ± 0.06 |
0.65 ± 0.09 |
0.11 ± 0.01 |
0.12 ± 0.00 |
| 50 |
0.56 ± 0.02 |
0.26 ± 0.07 |
0.11 ± 0.01 |
0.12 ± 0.01 |
| 100 | 0.77 ± 0.14 | 0.10 ± 0.01 | 0.11 ± 0.01 | 0.13 ± 0.01 |
*Each value is the mean of triplicates ± SD (ANCOVA), P. aeruginosa was incubated in BHI medium for 22 h in presence of different acid concentrations (acetic, lactic acid or HCl).