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. 2014 Apr 27;14:107. doi: 10.1186/1471-2180-14-107

Table 4.

Species assignation of Lactobacillus isolates based on MALDI-TOF analyses, 16S RNA and rpoA genes sequencing

Strain Mass spectrometry
 16S RNA gene sequencing
 rpoA gene sequencing
 API 50 CHL
Identification Score* Identification NCBI accession number (GenBank) Identification NCBI accession number (GenBank) Identification Score
ES.A.2
 L. fermentum
 1.916
 L. fermentum
 [KC762296]
 L. fermentum
 [KC861367]
 L. fermentum
 96.3%
ES.D.88
 L. paracasei
 2.458
 L. casei group
 [KC762297]
 L. paracasei
 [KC861369]
 L. paracasei
 98.4%
ES.F.115
 L. fermentum
 2.21
 L. fermentum
 [KC762298]
 L. fermentum
 [KC861368]
 L. fermentum
 98.5%
Od.76***
 L. zeae
 2.089
 L. casei group
 [KC762299]
 L. zeae/casei
 [KC861370]
 - **
- **
L. casei
 2.043
K.C6.3.1D
 L. fermentum
 2.18
 L. fermentum
 [KC762300]
 L. fermentum
 [KC861371]
 L. fermentum
 99.7%
K.C6.3.1E
 L. fermentum
 2.109
 L. fermentum
 [KC762301]
 L. fermentum
 [KC861372]
 L. fermentum
 99.7%
K.V9.3.2B
 L. fermentum
 2.163
 L. fermentum
 [KC762302]
 L. fermentum
 [KC861373]
 L. fermentum
 99.7%
K.V9.3.2C L. fermentum 2.223 L. fermentum [KC762303] L. fermentum [KC861374] L. fermentum 99.8%

*An isolate was considered well identified at the species level with a MALDI-TOF mass spectrometry score ≥ 1.9. **The API 50 CHL gallery is not designed to identify L. zeae. ***Od.76 was formally identified as a L. zeae by sequencing the pheS gene (Genbank accession number: KJ402364).