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. Author manuscript; available in PMC: 2014 Jun 2.
Published in final edited form as: J Cell Physiol. 2013 Dec;228(12):2337–2342. doi: 10.1002/jcp.24404

Figure 4. Differential chromatin accessibility on the ANP and BNP promoters in left ventricles from Dahl salt-sensitive and Dahl salt-resistant rats.

Figure 4

Male Dahl salt-sensitive (S) and salt-resistant (R) rats were given a 2% NaCl diet at 6 weeks of age for another 6 weeks. Nuclei were isolated from the left ventricles of S and R rats and digested with MNase I. Accessibility was quantified by qPCR by calculating (CT undigested-CT digested), normalizing to undigested samples and also by further normalizing to the values obtained with primers to a control region (the myelin protein zero intron) after 5 and 15 minutes of MNase treatment. A. Chromatin accessibility on the ANP promoter. B. Chromatin accessibility on the BNP promoter. Bars represent the average obtained from independent experiments performed with the indicated number of rat hearts (N). Error bars represent SEM.