Fig. 2.
The SMARCAL1 HARP domain can operate with the UvsW motor domain. (A) Design of a HARP-UvsW chimera, in which the N-terminal substrate recognition domain of UvsW (yellow) is replaced with HARP1 from SMARCAL1 (green). The RecA lobes of the ATPase domain are in red and blue. (B) Schematic of the four constructs tested, with domains colored as in A. The position of the K141R Walker A mutation, which impairs UvsW ATPase and fork regression activities, is denoted by a white X. (C) Fork regression activity of wild-type UvsW (yellow circles), HARP-UvsW chimera (green squares), HARP-UvsWK141R mutant (black triangles), and UvsWATPase domain (gray diamonds). Raw data are provided SI Appendix, Fig. S5.