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. 2014 May 12;111(21):7582–7587. doi: 10.1073/pnas.1406383111

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Fig. 4. — Cell-specific expression of endosperm genes as determined by in situ hybridization. (A) Expression in the AL only. Section of an 8-DAP kernel hybridized with a probe for GRMZM2G091054 (AL9). (B) Expression in the BETL only. Section of a 6-DAP kernel hybridized with a probe for AC199820.4_FG006 (ZmMYBR29). (C) Expression in the BIZ only. Section of an 8-DAP kernel hybridized with a probe for GRMZM2G406170 (BURP domain-containing). (D) Expression in the BETL and BIZ. Section of an 8-DAP kernel hybridized with a probe for GRMZM2G088896 (uncharacterized). (E) Close-up of the image in A showing expression localized to the AL. (F) Close-up of the image in B showing expression localized to the BETL. (G) Close-up of the image in C showing expression localized to the BIZ. (H) Close-up of the image in D showing expression localized in the BETL and BIZ. (I) Expression in the CZ only. Section of a 7-DAP kernel hybridized with a probe for GRMZM2G006585 (ZmPLATZ12). (J) Expression in the ESR only. Section of a 7-DAP kernel hybridized with a probe for GRMZM2G120008 (uncharacterized). (K) Expression in the ESR, BETL, and BIZ. Section of a 7-DAP kernel hybridized with a probe for GRMZM2G128832 (uncharacterized). (L) Expression in the ESR, BETL, BIZ, and CZ. Section of a 7-DAP kernel hybridized with a probe for GRMZM2G046532 (uncharacterized). (M) Expression in the SE and CZ. Section of a 10-DAP kernel hybridized with a probe for GRMZM2G127160 (tryptophan aminotransferase). (N) Expression in the SE only. Section of a 10-DAP kernel hybridized with a probe for GRMZM2G023872 (ZmGRAS20). (O) Close-up of the image in N. (P) Negative control. Section of a 6-DAP kernel hybridized with a probe for GRMZM2G453555 (transposable element), which does not produce a signal. (Scale bars: 2 mm for A, B, C, D, G, I, J, K, L, M, N, and P; 500 µm for E, F, H, O). al, aleurone; betl, basal endosperm transfer layer; biz, basal intermediate zone; cz, conducting zone; emb, embryo; esr, embryo surrounding region; se, starchy endosperm.