Fig. 2.

The effects of IDDs and RGA on the expression of SCL3. (A and B) A transient reporter assay was used to examine the transactivation effects of IDDs and RGA on the expression of SCL3 in protoplasts from Arabidopsis T87 suspension-cultured cells. (A) Schematic representations of the effector and reporter constructs. (B) Bars marked −RGA represent experiments in which the reporter, IDD effector plasmid, and empty RGA effector plasmids were cotransfected. Bars marked +RGA correspond to experiments in which each of the IDDs, RGA, and reporter plasmids were cotransfected. As a negative control, an empty effector vector (vec) was used in place of the IDD plasmids. The relative activity caused by vector control (far left bar) was set as 1. Results represent the means of three experiments; error bars represent SD. En. 35S, enhanced 35S promoter whose details are described in SI Materials and Methods. (C) Transient reporter assay in cultured cell protoplast to determine where AtIDD3 exerts its effect on the SCL3 promoter. Schematic representations of the effector construct, IDD3-VP16, and SCL3 promoter deletion are shown on the left. (D) Transient reporter assays using AtIDD3, RGA, and the −183 and −100 SCL3 promoter constructs. The AtIDD3 and RGA constructs were cotransfected as effectors. The relative activity caused by vector control was set as 1.